Scientific journal
Название журнала на английском
ISSN 2500-0802
ПИ №ФС77-61154

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CONCENTRATION DEPENDENCE OF THE VISCOSITY OF PROTEIN SYSTEMS AND REFRACTOMETRIC ANALYSIS PROTEIN SOLUTION

Minyaeva O.A. 1 Botova D.I. 1 Nelyubina E.S. 1
1 South Ural State Medical University
Empirically found that the concentration dependence of the kinematic, dynamic and relative viscosity of solutions of globular proteins with spherical globules (albumin and interferon alfa human leukocyte) submit to linear laws. This allows us to apply the classical Einstein’s equation ηотн = k + α·С to describe the patterns of change in viscosity in protein solutions adjusted to the physical meaning and values of the coefficients. If the protein globules anisodiametric (normal immunoglobulin solutions), the linear correlation between concentration and viscosity is broken. The concentration dependence of the viscosity in this case is satisfactorily approximated by a polynomial of second degree. The calculated values of the coefficients in the Einstein equation for albumin and interferon alfa-human leukocyte distinct from the classical and reflect the influence of the level of intermolecular interactions in solution, protein molecular weight, shape and size of the protein globules on the viscosity of aqueous solutions. Adding albumin and interferon alfa-human leukocyte leads to a linear change in the refractive index of the solution with increasing protein concentration.