Scientific journal
Название журнала на английском
ISSN 2500-0802
ПИ №ФС77-61154

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THE FORMATION OF THE PIEZOELECTRIC IMMUNOSENSOR’S RECEPTOR LAYER FOR THE DETECTION OF TRACE CONCENTRATIONS OF RACTOPAMINE

Vasilev S.V. 1 Popova M.V. 1 Farafonova O.V. 1 Karaseva N.A. 1 Ermolaeva T.N. 1
1 Lipetsk State Technical University
The conditions of the nanostructured affine layer’s formation on the surface of the gold electrode of the piezoelectric gravimetric sensor intended for highly sensitive and selective detection of trace concentration of beta-agonist (ractopamine) in liquid environments have been experimentally studied and theoretically proved. The conditions of creating of a substrate on the basis of the self-organized monolayers of thiols, silanes have been studied. The conjugates of ractopamine with protein molecules were immobilized on the surface of substrate by the activation with bifunctional reagents. The way of modification of a gold electrode’s surface of the piezoelectric sensor was suggested in consideration of the value of concentration sensitivity, weight and stability of the recognizing layer. This way makes it possible to detect the presence of ractopamine in test sample at the level of 0,05 ng/ml and to carry out its definition in the range of 0,1 - 1 ng/ml.